M.Sc (Botony), M.Phil (Botony), The Global Open University, Chumoukedima, Nagaland
JournalPIJST
Volume / Issue2 / 4
Pages1–4
Published30 Apr 2025
Paper IDPIJST24A25001
Views / Downloads1 / 0
Article summary
Abstract
Secondary metabolites of plant origin—alkaloids, terpenoids, phenolics, and naphthoquinones—remain indispensable leads and adjuvants in modern therapeutics. Conventional field cultivation faces bottlenecks (low yields, seasonal/geographical variability, overharvesting). In vitro plant cell, tissue, and organ culture platforms offer controlled, year-round production and enable targeted pathway manipulation, aligning well with biomedical pipelines that require batch-to-batch consistency, traceability, and cGMP-amenable scale-up. This paper synthesizes current methods for producing high-value metabolites using callus and cell suspensions, organ cultures (shoots, embryos), and Agrobacterium rhizogenes–induced hairy roots. We review elicitation and process optimization (biotic/abiotic cues, precursor feeding, medium design, light/pH), metabolic engineering (pathway genes, transcription factors, CRISPR), bioreactor strategies (stirred-tank, wave, air-lift, disposable systems), and downstream analytics (extraction; HPLC/LC–MS quantification). Case studies cover clinically relevant molecules and their biomedical contexts: paclitaxel from Taxus systems (oncology), artemisinin from Artemisia annua (antimalarial), vincristine/vinblastine from Catharanthus roseus (oncology), and shikonin from Lithospermum erythrorhizon (anti-inflammatory/wound healing). We highlight translational links to animal models (zebrafish, murine xenografts, toxicity profiling) and discuss techno-economics, sustainability, and regulatory considerations. Finally, we identify challenges—genetic drift, scale-dependent oxygen transfer, elicitation reproducibility—and propose future directions including systems-guided design, single-use intensified bioprocesses, and hybrid plant–microbial routes. Collectively, plant cell/organ factories can de-risk supply chains for essential medicines while reducing pressure on biodiversity, provided that engineering control, rigorous analytics, and quality systems converge.
R.M. Asha (2025). In Vitro Production of Secondary Metabolites through Plant Cell and Organ Cultures (with Biomedical Relevance). Procedure International Journal of Science and Technology, 2(4), 1–4. https://www.pijst.com/article/pijst24a25001/in-vitro-production-of-secondary-metabolites-through-plant-cell-and-organ-cultures-with-biomedical-relevance
R.M. Asha. “In Vitro Production of Secondary Metabolites through Plant Cell and Organ Cultures (with Biomedical Relevance).” Procedure International Journal of Science and Technology, vol. 2, no. 4, 2025, pp. 1–4. https://www.pijst.com/article/pijst24a25001/in-vitro-production-of-secondary-metabolites-through-plant-cell-and-organ-cultures-with-biomedical-relevance
R.M. Asha. “In Vitro Production of Secondary Metabolites through Plant Cell and Organ Cultures (with Biomedical Relevance).” Procedure International Journal of Science and Technology 2, no. 4 (2025): 1–4. https://www.pijst.com/article/pijst24a25001/in-vitro-production-of-secondary-metabolites-through-plant-cell-and-organ-cultures-with-biomedical-relevance
R.M. Asha (2025) ‘In Vitro Production of Secondary Metabolites through Plant Cell and Organ Cultures (with Biomedical Relevance)’, Procedure International Journal of Science and Technology, 2(4), pp. 1–4. Available at: https://www.pijst.com/article/pijst24a25001/in-vitro-production-of-secondary-metabolites-through-plant-cell-and-organ-cultures-with-biomedical-relevance.
R.M. Asha, “In Vitro Production of Secondary Metabolites through Plant Cell and Organ Cultures (with Biomedical Relevance),” Procedure International Journal of Science and Technology, vol. 2, no. 4, pp. 1–4, 2025. https://www.pijst.com/article/pijst24a25001/in-vitro-production-of-secondary-metabolites-through-plant-cell-and-organ-cultures-with-biomedical-relevance.
R.M. Asha. In Vitro Production of Secondary Metabolites through Plant Cell and Organ Cultures (with Biomedical Relevance). Procedure International Journal of Science and Technology. 2025;2(4):1–4. https://www.pijst.com/article/pijst24a25001/in-vitro-production-of-secondary-metabolites-through-plant-cell-and-organ-cultures-with-biomedical-relevance.
R.M. Asha. In Vitro Production of Secondary Metabolites through Plant Cell and Organ Cultures (with Biomedical Relevance). Procedure International Journal of Science and Technology 2025, 2 (4), 1–4. https://www.pijst.com/article/pijst24a25001/in-vitro-production-of-secondary-metabolites-through-plant-cell-and-organ-cultures-with-biomedical-relevance.
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